Involvement of multiple proteases during Fas-mediated apoptosis in T lymphocytes
- 8 May 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 364 (2) , 134-138
- https://doi.org/10.1016/0014-5793(95)00370-o
Abstract
The mechanism of Fas antigen-mediated apoptosis is at present unclear. We show here that the100,000 × g supernatant from cell lysates prepared from anti-Fas-stimulated JURKAT T cells, induces chromatin fragmentation in isolated nuclei with concomitant morphological changes typically seen in apoptosis. The formation of this apoptotic nuclei promoting activity (ANPA) in JURKAT T cells after Fas antigen ligation was blocked by the serine protease inhibitors, TPCK and DCI, and by the interleukin 1-β-converting enzyme inhibitor, VAD-FMK. In addition, chromatin degradation and morphological changes mediated by the ANPA in isolated nuclei were inhibited by TPCK, but not by DCI or VAD-FMK. These results suggest that Fas-mediated apoptosis in T cells involves the activation of a cascade of proteases.Keywords
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