.ALPHA.-Galactosidase from alfalfa seeds (Medicago sativa L.).
- 1 January 1979
- journal article
- Published by Oxford University Press (OUP) in Agricultural and Biological Chemistry
- Vol. 43 (7) , 1499-1504
- https://doi.org/10.1271/bbb1961.43.1499
Abstract
An α-galactosidase from alfalfa seeds was purified 140-fold by ammonium sulfate frac-tionation, and column chromatography on Sephadex G-100, DEAF- and CM-Sephadex. Polyacrylamide-gel electrophoresis of the purified enzyme showed a single protein band. The molecular weight was estimated to be approximately 57, 000 by gel-filtration. The purified enzyme hydrolyzed p-nitrophenyl α-D-galactoside more rapidly than raffinose. The maximal enzyme activities were obtained at pH 4.0 and 5.5 for p-nitrophenyl α-D-galactoside and at 4.5 for raffinose. The enzyme was shown to be inhibited by Hg2+ and Ag+ ions, and D-galactose.Keywords
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