Detection of Tomato Yellow Leaf Curl Virus in Lysates of Plants and Insects by Hybridization with a Viral DNA Probe
- 1 January 1988
- journal article
- research article
- Published by Scientific Societies in Plant Disease
- Vol. 72 (11) , 949-951
- https://doi.org/10.1094/pd-72-0949
Abstract
Tomato yellow leaf curl virus (TYLCV) was diagnosed in tomato plants in insect vectors by use of a virus-specific probe. By molecular hybridization with radiolabeled TYLCV DNA, we detected viral nucleic acids in lysates prepared from infected tomato plant tissues such as leaves, roots, stems, shoot apex, and cotyledons and showed that the virus is not evenly distributed throughout the plant. The TYLCV probe also detected the virus in lysates of individual whitefly (Bemisia tabaci) vectors. The TYLCV-specific DNA probe can serve as a powerful tool in monitoring the tomato yellow leaf curl disease.This publication has 1 reference indexed in Scilit:
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977