Measurement of Antidepressants Using Solid-Phase Extraction and Wide-Bore Capillary Gas Chromatography with Nitrogen-Selective Detection

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Abstract
A comprehensive method is presented for the determination of nine antidepressant drugs and metabolites in serum: amitriptyline, nortriptyline, imipramine, desipramine, maprotiline, doxepin, desmethyldoxepin, protriptyline, and trimipramine. Chlorimipramine is used as the internal standard. A simple solid-phase extraction procedure utilizing disposable reversed-phase C18 columns is described. Samples are analyzed by gas chromatography with nitrogen-selective detection using a wide-bore capillary column with a permanently bonded, non-polar stationary phase. The assay possesses linearity to 800 ng/mL for maprotiline and 500 ng/mL for the other antidepressants, sensitivity to at least 25 ng/mL, recovery ranging from 96 to 107%, and between-run precision reflected by CVs of 4.4 to 8.1%. Lack of interference is documented for over 27 commonly prescribed prescribed drugs. We conclude that the method reported here is idealy suited for monitoring therapeutic and toxic levels of antidepressant drugs.

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