From quantitative microscopy to automated image understanding
Open Access
- 1 January 2004
- journal article
- research article
- Published by SPIE-Intl Soc Optical Eng in Journal of Biomedical Optics
- Vol. 9 (5) , 893-913
- https://doi.org/10.1117/1.1779233
Abstract
Biomedical research has been revolutionized by the new types of information generated from various “omics” projects, beginning with the genome sequencing projects. The genome drafts completed so far have enabled us, for the first time, to discover and compare all possible genes in a number of organisms. To uncover proteome differences in a given organism, expression arrays and protein chips have been used to study the transcription and expression characteristics of all possible proteins in different tissues, at different developmental stages, and under various disease types.1 2 High-throughput pipelines in structural proteomics have automated protein structure determination by integrating target purification, crystallization, data acquisition, and final assignment.3 Location proteomics, one of the latest subfields of proteomics, has the goal of providing an exact description of the subcellular distribution for each protein in a given cell type.4 5 6 7 All of these methods provide valuable information for determining how a protein functions and how its functioning is regulated.Keywords
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