The Assimilatory and Dissimilatory Nitrate Reductases of Pseudomonas aeruginosa are Encoded by Different Genes

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Abstract
The phenotypes of certain mutant strains of P. aeruginosa were reported to be pleiotropic for nitrate reduction; these strains were selected for their inability to dissimilate nitrate and also lost the ability to assimilate nitrate. The isolation procedure selected 2 mutations, one in genes encoding the synthesis of dissimilatory nitrate reductase (narA, narB or narE) and another in one of the genes (nas) encoding the synthesis of assimilatory nitrate reductase. Thus in P. aeruginosa dissimilatory and assimilatory nitrate reductases are genetically distinct. However, a loss of both enzymes is necessary to prevent slow dissimilatory growth on nitrate. Assimilatory nitrate reductase requires Mo to function, as does dissimilatory nitrate reductase. Lesions in narD affect incorporation of Mo into both enzymes, and hence exert a pleiotropic effect.

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