Anti‐HLA‐A2 and ‐A28 monoclonal antibody: production and study of the cross‐reaction
- 1 May 1984
- journal article
- research article
- Published by Wiley in Tissue Antigens
- Vol. 23 (5) , 274-279
- https://doi.org/10.1111/j.1399-0039.1984.tb00045.x
Abstract
An anti-HLA-A2 and -A28 monoclonal antibody, XV.17, has been prepared by immunizing a Balb/c mouse with PBL. This XV.17 monoclonal antibody is a cytotoxic IgM. Its reactivity was tested by lymphocytotoxicity test and indirect immunofluorescence technique, in parallel with an alloantiserum ORA having the same anti-HLA-A2, -A28 reactivity pattern, against different panels. Family studies were undertaken. Absorptions-elutions and cytofluorometry experiments were performed to study the cross-reaction. The XV. 17 monoclonal antibody is cytotoxic against all the HLA-A2 and -A28 tested cells, and is absorbed by HLA-Aw23 and -Aw24 cell susepnsions.This publication has 26 references indexed in Scilit:
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