VITAMIN REQUIREMENTS FOR LIPASE PRODUCTION BY CANDIDA LIPOLYTICA

Abstract
A culture of C. lipolytica, while grown successfully on a defined synthetic medium containing no added vitamins, failed to produce a measurable amt. of lipase in 72 hrs. Additions to the above medium of biotin, nicotinic acid, pantothenic acid and 4-methyl-5-beta-hydroxyethyl thiazole increased growth but failed also to induce lipase production. Both thiamine chloride and 2-methyl-5-bromomethyl-6-aminopyrimidine dihydrobromide enhanced growth and also induced lipase production by the organisms.

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