Separation and purification of blood plasma arginine vasopressin on Sephadex G-50M for subsequent radioimmunoassay

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Abstract
A gel filtration method was developed for purification and separation of plasma arginine-vasopressin (AVP) from proteins interfering with RIA[radioimmunoassay]-determination of AVP. The method involves filtration of 1 ml of [human, dog, sheep, goat] plasma on a Sephadex G-50M gel column and the eluant used is 100 mM borate buffer. This rapid, and relatively simple method gives rather complete separation of plasma protein and AVP with an AVP recovery of .apprx. 90% at normal or elevated plasma AVP levels. Since 30 plasma samples may be run simultaneously, the method has potential value for routine laboratory use.