Catabolite-Insensitive Revertants of Lac Promoter Mutants
- 1 July 1970
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 66 (3) , 773-779
- https://doi.org/10.1073/pnas.66.3.773
Abstract
The maximum rate of expression of the lac operon is severely reduced in lac promoter mutants. Revertants of these mutations which produce higher levels of enzyme were isolated. Some of these revertants had lost sensitivity to catabolite repression and transient repression. The mutations responsible for these losses took place at sites very close to the original promoter mutations. From these results we conclude that the promoter itself is the target site for both catabolite and transient repression of the lac operon.Keywords
This publication has 15 references indexed in Scilit:
- Genetic Control of Histidine Degradation in Salmonella typhimurium, Strain LT-2Journal of Biological Chemistry, 1969
- Cyclic AMP regulates Catabolite and Transient Repression in E. coliNature, 1969
- The step sensitive to catabolite repression and its rever sal by 3′–5′ cyclic AMP during induced synthesis of β-galactosidase in E. coliBiochemical and Biophysical Research Communications, 1969
- THE STIMULATORY EFFECT OF CYCLIC ADENOSINE 3′5′-MONOPHOSPHATE ON DNA-DIRECTED SYNTHESIS OF β-GALACTOSIDASE IN A CELL-FREE SYSTEMProceedings of the National Academy of Sciences, 1969
- Catabolite Sensitive Site of the lac OperonNature, 1969
- Molecular basis of transient repression of beta-galactosidase in Escherichia coli.1969
- The nature of mutants in the lac promoter regionJournal of Molecular Biology, 1968
- The role of the lac promotor locus in the regulation of beta-galactosidase synthesis by cyclic 3',5'-adenosine monophosphate.Proceedings of the National Academy of Sciences, 1968
- Induction and Repression of the Histidine-degrading Enzymes of Bacillus subtilisJournal of Biological Chemistry, 1968
- New Controlling Element in the Lac Operon of E. coliNature, 1968