Lack of Hemocyte Chemiluminescence Stimulation byPerkinsus marinusin Eastern OystersCrassostrea virginicawith Dermo Disease
- 1 June 1999
- journal article
- Published by Oxford University Press (OUP) in Journal of Aquatic Animal Health
- Vol. 11 (2) , 179-182
- https://doi.org/10.1577/1548-8667(1999)011<0179:lohcsb>2.0.co;2
Abstract
Phagocytosis of zymosan A particles by hemocytes of eastern oyster Crassostrea virginica stimulates the production of reactive oxygen species (ROS), which are easily quantified by luminol‐augmented chemiluminescence (CL). Antimicrobial defense mechanisms of hemocytes may involve the activity of cytotoxic ROS. The CL response to phagocytosis of zymosan by hemocytes from C. virginica with advanced Perkinsus marinus infections is more elevated than that produced by zymosan in cells from uninfected oysters. This effect is perhaps akin to macrophage activation. Phagocytosis of P. marinus cells by hemocytes withdrawn from uninfected oysters produced no detectable CL response. Hemocytes withdrawn from oysters with P. marinus infections ranging from light to heavy were evaluated for CL responses after phagocytosis of zymosan or P. marinus. Increases in CL stimulation by zymosan were seen as the intensity of infection increased. Despite avid phagocytosis of P. marinus, CL activity of the hemocytes was not seen, regardless of the level of infection of the host. Lack of hemocytic ROS stimulation by ingestion of P. marinus cells may contribute to in vivo survival of this parasite.Keywords
This publication has 14 references indexed in Scilit:
- Perkinsus marinussecretory products modulate superoxide anion production by oyster (Crassostrea virginica) haemocytesFish & Shellfish Immunology, 1999
- In vitro interaction of Perkinsus marinus merozoites with eastern and pacific oyster hemocytesDevelopmental & Comparative Immunology, 1995
- Defense Responses of Hemocytes Withdrawn from Crassostrea virginica Infected with Perkinsus marinusJournal of Invertebrate Pathology, 1995
- Hemocyte-derived reactive oxygen intermediate production in four bivalve mollusksDevelopmental & Comparative Immunology, 1994
- Continuous in Vitro Culture of the Eastern Oyster Parasite Perkinsus marinusJournal of Invertebrate Pathology, 1993
- Perkinsus marinus susceptibility and defense-related activities in eastern oysters Crassostrea virginica. temperature effectsDiseases of Aquatic Organisms, 1993
- Increased Reactive Oxygen Intermediate Production by Hemocytes Withdrawn from Crassostrea virginica Infected with Perkinsus marinusThe Biological Bulletin, 1992
- Localization and characterization of acid phosphatase activity in Bonamia ostreae (Ascetospora), an intrafemocytic protozoan parasite of the flat oyster Ostrea edulis (Bivalvia)Diseases of Aquatic Organisms, 1992
- 10.3 Chemiluminescent responses of Ostrea edulis and Crassostrea gigas hemocytes to Bonamia ostreae (Ascetospora)Developmental & Comparative Immunology, 1989
- The Role of Lysosomes in Molluscan Inflammation1American Zoologist, 1983