Characterization of Xylansulfate by Size Exclusion Chromatography

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Abstract
A sulfated, low molecular weight beech xylan was fractionated preparatively on Sephadex G-50 into ten fractions with molecular weights from 17 000 to 1 800. The degree of sulfation, refractive index increment, molecular weight, and specific optical rotation of each fraction was determined. The fractions were then used to calibrate a Sephacryl S–200 gel column for the molecular weight distribution analysis of xylansulfate. The molecular weights, M W, obtained by SEC for samples of xylansulfate were in good agreement (within 4 ± 1%) to the values derived by low angle light scattering.