Formation and Liberation of Zoospores in Isoachlya anisospora var. indica

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Abstract
During the development of the sporangium, the stage of inflow of the protoplasm is successively followed by the stage of preliminary division, "the homogeneous stage," and the stage of final division and the formation of zoospores. The vacuolar system has been studied in all the stages by growing the fungus in a medium supplemented by neutral red. No nuclear division is observed within the sporangium. Mitochondria are present as rod-shaped and granular structures in all the stages, but in the germ tubes rod-shaped and filamentous forms are seen. The opt. temp. for the formation of sporangia lies near 25[degree]C. They are formed in media having pH values between 4.5 and 9. More acidic and alkaline media inhibit their formation.