Transcription factor USF from duck erythrocytes transactivates expression of the histone H5 genein vitroby interacting with an intragenic sequence
- 1 January 1990
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 18 (5) , 1225-1231
- https://doi.org/10.1093/nar/18.5.1225
Abstract
The duck histone H5 gene contains a 12 base pair (bp) sequence motif within the coding region, which shows homology in 10 out of 12 bp with the consensus sequence of the USF binding site in the Ad2ML-promoter. The functional equivalent of transcription factor USF, partially purified from whole cell extracts of duck erythrocytes (EUSF), was shown to interact with this intragenic sequence. Electrophoretic mobility shift analyses revealed the selective formation of a complex between this protein fraction and the duck H5 gene. Footprint assays with DNase I delineated specific binding to the intragenic sequence outlined above. Moreover this protein fraction, containing EUSF, transactivates the expression of the duck H5 gene in vitro and elimination of the USF-consensus sequence leads to a loss of stimulation but retains the basic transcription of the gene. These results suggest an as yet unknown functional role of EUSF in the expression of the H5 gene of the duck.Keywords
This publication has 31 references indexed in Scilit:
- A region internal to the coding sequences is essential for transcription of the yeast Ty-D15 element.Molecular and Cellular Biology, 1989
- Erythroid-specific transcription of the chicken histone H5 gene is directed by a 3′ enhancerNature, 1987
- Purified transcription factor AP-1 interacts with TPA-inducible enhancer elementsCell, 1987
- Isolation of a Saccharomyces cerevisiae centromere DNA-binding protein, its human homolog, and its possible role as a transcription factor.Molecular and Cellular Biology, 1987
- A 3′ enhancer is required for temporal and tissue-specific transcriptional activation of the chicken adult β-globin geneNature, 1986
- Identification of a cyclic-AMP-responsive element within the rat somatostatin gene.Proceedings of the National Academy of Sciences, 1986
- Downstream sequences affect transcription initiation from the adenovirus major late promoter.Molecular and Cellular Biology, 1986
- Fine analysis of the active H5 gene chromatin of chicken erythroid cells at different stages of differentiationJournal of Molecular Biology, 1986
- Protein factor(s) binding independently to two different regions of the adenovirus 2 major late promoterNucleic Acids Research, 1986
- An RNA polymerase II transcription factor binds to an upstream element in the adenovirus major late promoterCell, 1985