DYEBINDING AND MUCOPOLYSACCHARIDE CONTENT IN CONNECTIVE TISSUE

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Abstract
The hyaluronic acid content of the mucoid layer of the rooster comb and the chondroitin sulfuric acid content of bovine nasal cartilage were determined in the fresh tissue, in the water extract of the tissue, and in the water-extracted residue. The binding of cationic dye by the tissue, the tissue extract and the residue was compared with the mucopolysaccharide content. The molar ratio of the bound dye to the anionic sites of the mucopolysaccharides varied between 1.1 and 1.8. In the comb extract this ratio was one. This indicates that, with the exception of the comb extract, anionic sites other than those of the mucopolysaccharides are also responsible for the dyebinding.