CRYOSURVIVAL OF RAM SPERMATOZOA IN HYPERTONIC AND ISOTONIC DILUENTS
- 1 June 1982
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Animal Science
- Vol. 62 (2) , 425-428
- https://doi.org/10.4141/cjas82-049
Abstract
The cryosurvival (percentage of motile spermatozoa and rate of foward progression) of ram spermatozoa after freezing in hypertonic and isotonic diluents containing a buffered dextran-sugar combination or buffered dextran-sugar plus egg yolk was compared immediately after thawing and after incubation at 39° for 60 min. Cryosurvival of spermatozoa was comparable in both diluents. However, the cryosurvival of spermatozoa was increased significantly in hypertonic diluents compared with that in isotonic diluents, regardless of their composition. Key words: Spermatozoa, sheep, cryosurvival, diluentsThis publication has 7 references indexed in Scilit:
- Effect of osmolality of skim-milk diluents and thawing rate on cryosurvival of ram spermatozoaCryobiology, 1981
- An evaluation of cryoprotective compounds on bovine spermatozoaCryobiology, 1980
- Fertility in the ewe after artificial insemination with fresh and frozen semen at the induced oestrus, and influence of the photoperiod on the semen quality of the ramLivestock Production Science, 1979
- Differing actions of penetrating and nonpenetrating cryoprotective agentsCryobiology, 1978
- The distribution of cryoprotective agents into lipid interfacesCryobiology, 1977
- Improved method for the cryopreservation of human red cells in liquid nitrogen with hydroxyethyl starchCryobiology, 1976
- Components in Egg Yolk which Protect Bovine Spermatozoa during FreezingJournal of Animal Science, 1974