Studies on Lyophiled Cultures

Abstract
Legume nodule bacteria (R. leguminosarum) originally isolated from alfalfa, lespedeza, cowpea, pea, soybean, vetch, crown vetch, and red clover were lyophiled and preserved for periods of 31/2-4 yrs., after which their ability to grow, nodulate suitable host plants and fix N2 was detd. Regularly transferred stock cultures from which the lyophiled cultures were taken were used for comparison. No marked difference between lyophiled and stock cultures was observed when judged by the number of nodules and by the color of the plants.

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