Microbial Growth Comparisons of Five Commercial Parenteral Lipid Emulsions

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Abstract
The ability of parenteral lipid emulsions to support microbial growth was compared using commercially available brands of lipid emulsion. Both 10 and 20% concentrations of soybean and safflower oil emulsions were used. Washed cultures of six gram-negative, three gram-positive, and one yeast, in concentrations of 1 × 104 to 2 × 104 colony-forming units/ml, were inoculated into lipid emulsion aliquots and stored at room temperature. These were then subcultured at 0, 6, 12, 24 and 48 hr. After 48 hr at 37°C, growth was recorded as colony-forming units/ml. Normalized growth curves were expressed as x ± SEM. ANOVA demonstrated no difference in growth patterns due to the nature of the oil or its concentration. Gram-negative organisms multiplied faster when compared to gram-positive (p < 0.05 at 12 hr, p < 0.01 at 24 hr, and p < 0.005 at 48 hr). Yeast grew as well as bacteria. The Center for Disease Control's recommendation of a 12-hr hang time for parenteral lipid emulsions should be observed until correlation of laboratory microbial growth patterns and clinical use are studied further. (Journal of Parenteral and Enteral Nutrition 8:391-395, 1984)

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