Physical map of polyoma viral DNA fragments produced by cleavage with a restriction enzyme from Haemophilus aegyptius, endonuclease R-HaeIII
Open Access
- 1 April 1975
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 15 (4) , 946-953
- https://doi.org/10.1128/jvi.15.4.946-953.1975
Abstract
Digestion of polyoma viral DNA with a restriction enzyme from Haemophilus aegyptius generates at least 22 unique fragments. The fragments have been characterized with respect to size and physical order on the polyoma genome, and the 5' to 3' orientation of the (+) and (-) strands has been determined. A method for specific radiolabeling of adjacent fragments was employed to establish the fragment order. This technique may be useful for ordering the fragments produced by digestion of complex DNAs.Keywords
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