Nonradioactive DNA probe for detection of gene for methicillin resistance in Staphylococcus aureus
Open Access
- 1 March 1991
- journal article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 35 (3) , 575-578
- https://doi.org/10.1128/aac.35.3.575
Abstract
A DNA probe was developed by inserting, in the SmaI site of pBluescript sK, a 528-bp fragment of the gene responsible for intrinsic resistance to beta-lactam antibiotics in Staphylococcus aureus (mec determinant). The mec probe provided a useful tool for the rapid identification of the intrinsic resistance trait and for establishing guidelines for testing the in vitro susceptibility of S. aureus to beta-lactams.Keywords
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