DNA Synthesis in Human Fibroblasts: Stimulation by Insulin and by Nonsuppressible Insulin-like Activity (NSILA-S)
- 1 September 1974
- journal article
- research article
- Published by The Endocrine Society in Journal of Clinical Endocrinology & Metabolism
- Vol. 39 (3) , 512-521
- https://doi.org/10.1210/jcem-39-3-512
Abstract
The effects of acid-soluble nonsuppressible insulin-like activity (NSILA-S) and insulin on DNA synthesis by cultured human fibroblasts have been compared. NSILA-S and insulin, like serum, stimulated [3H]thymidine incorporation into DNA after an 8- to 10-hour lag, reaching maximum levels after 16 to 20 hr. Insulin was effective only at high concentrations, 0.02 μg/ml or greater, whereas NSILA-S was active at concentrations that have been reported in human serum. Combined addition of maximally effective concentrations of insulin and NSILA-S failed to give additive stimulation, suggesting that the two polypeptides act by a common mechanism. In contrast, combined addition of insulin and serum, or NSILA-S and serum, gave additive responses.Keywords
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