PROCEDURE FOR PROCESSING OF CENTRAL NERVOUS-SYSTEM TISSUE FOR IMMUNOFLUORESCENCE, LIGHT, AND ELECTRON-MICROSCOPIC EVALUATION
- 1 January 1978
- journal article
- research article
- Vol. 39 (12) , 1946-1949
Abstract
A rapid and simple procedure was developed for the simultaneous localization of canine distemper encephalomyelitis viral antigen in CNS tissues by immunofluorescence and concomitant correlation with viral induced lesions in the contralateral brain hemisphere. Dog brain (1/2) was excised under deep peneral anesthesia and samples were collected for immunofluorescence by snap freezing in liquid N2. The other 1/2 brain was perfused under pressure with a buffered glutaraldehyde-paraformaldehyde mixture for subsequent light microscopic and ultrastructural evaluation. The technique was useful in studying CNS diseases in large outbred experimental subjects in cases where rodent tissue processsing was not feasible.This publication has 4 references indexed in Scilit:
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- Perfusion‐fixation of the brain with chrome‐osmium solutions for the rapid Golgi methodJournal of Anatomy, 1966
- PARAFFIN EMBEDDING TECHNIQUES FOR IMMUNOFLUORESCENT DEMONSTRATION OF NEUROTROPIC VIRUSES1966
- A PARAFFIN EMBEDDING TECHNIQUE FOR STUDIES EMPLOYING IMMUNOFLUORESCENCEJournal of Histochemistry & Cytochemistry, 1962