Immunohistochemical studies on the distribution of neuropeptides and serotonin in the suprachiasmatic nucleus of the Brattleboro rat.
- 1 January 1987
- journal article
- research article
- Published by International Society of Histology & Cytology in Archivum histologicum japonicum
- Vol. 50 (1) , 1-14
- https://doi.org/10.1679/aohc.50.1
Abstract
The distribution of vasoactive intestinal polypeptide (VIP), gastrin-releasing peptide (GRP)/bombesin, somatostatin, vasopressin, neuropeptide Y (NPY) and serotonin was examined immunohistochemically in the suprachiasmatic nucleus (SCN) of male rats genetically deficient for vasopressin (Brattleboro strain). VIP-containing neurons and their varicose fibers were preferentially distributed in large numbers in the ventromedial part of the SCN. GRP/bombesin-containing neurons and their fibers were also gathered in the ventral part of the SCN, particularly in the ventromedical region of the nucleus. Somatostatin-containing neurons and their fibers were prominent in the rostral and middle portions of the SCN, where the highest concentration of immunoreactivity was restricted in their ventromedial part. No vasopressin-immunoreactivity was found at all throughout the SCN. Profuse NPY-containing varicose fibers were observed in the ventrolateral part of the SCN, but no immunoreactive neurons were distributed in this nuclear region. Serotonergic fibers showed a topographic arrangement in the SCN: a serotonin-immunoreactive nerve plexus was predominantly distributed in the ventrolateral part. These findings indicate that the SCN of Brattleboro rats is composed of distinct subdivisions of immunoreactive cell bodies and fibers. The distribution of the five peptides and indoleamine within the SCN in the Brattleboro strain was compared with that in normal Long-Evans rats. Furthermore, both strains of rats were exogeneously administered with arginine-vasopressin, but no conspicuous difference in the regional patterns of immunoreactivity was detected. The possible role of vasopressin in the SCN is discussed.This publication has 22 references indexed in Scilit:
- GABA Neuron Systems in Hypothalamus and the Pituitary GlandNeuroendocrinology, 1982
- Novel Peptide Neuronal System in Rat Brain and PituitaryScience, 1981
- Distribution of serotonin-immunoreactivity in the central nervous system of the rat—Cell bodies and terminalsNeuroscience, 1981
- Identification of parvocellular vasopressin and neurophysin neurons in the suprachiasmatic nucleus of a variety of mammals including primatesJournal of Comparative Neurology, 1980
- Vasoactive intestinal polypeptide (VIP) in mouse and rat brain: An immunocytochemical studyBrain Research, 1980
- VIP in the rat brain: Evidence for a major pathway linking the amygdala and hypothalamus via the stria terminalisHistochemistry and Cell Biology, 1980
- Immunocytochemical localization of somatostatin-containing neurons in the rat hypothalamusCell and tissue research, 1979
- Projections from the parvocellular vasopressin‐ and neurophysin‐containing neurons of the suprachiasmatic nucleusJournal of Anatomy, 1978
- The role of suprachiasmatic nuclei of the hypothalamus in the production of circadian rhythmBrain Research, 1976
- Morphology of the Neurosecretory System in Rats Homozygous and Heterozygous for Hypothalamic Diabetes Insipidus (Brattleboro Strain)Endocrinology, 1965