Aescin Content in Embryogenic Callus and in Embryoids from Leaf Explants ofAesculus hippocastanum

Abstract
HPLC determinations of the aescin contents in calli and embryoids from leaf explants of Aesculus hippocastanum L. were carried out in order to determine whether it was possible to obtain aescin-forming proliferations in vitro. The results indicate that embryogenic calli and embryoids derived from them produce an amount of active principle higher than that of horse-chestnut seeds. The data are discussed in terms of the relation between tissue differentiation and secondary metabolites biosynthesis.

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