Either one of the two yeast EF-1? genes is required for cell viability
- 1 January 1985
- journal article
- research article
- Published by Springer Nature in Current Genetics
- Vol. 9 (8) , 693-697
- https://doi.org/10.1007/bf00449823
Abstract
Summary Two genes,TEF1 andTEF2, encode the protein elongation factor EF-1α in the yeastSaccharomyces cerevisiae. We have generated yeast haploid strains containing eitherTEF1 orTEF2 interrupted by insertion of a large piece of foreign DNA. Cells which contain either one functional copy of the EF-1α genes are viable. In contrast, attempts to isolate a yeast haploid strain with bothTEF1 andTEF2 inactivated have failed suggesting that the double gene disruption is a lethal event.Keywords
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