On the Structure and Staining of Starch Grains of the Potato Tuber

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Abstract
Procedures are descr. for the differential staining of starch grains of the potato tuber with hematoxylin, and for double staining with safranin O and fast green FCF. The staining effects obtained are made possible by the action of a swelling agent. Staining with hematoxylin is preceded by the swelling action of formaldehyde. In staining with safranin O and fast green FCF, the formaldehyde is added to the staining soln. The results obtained are as follows (1) a clavate-shaped, central struct. composed of small particles arranged in definite layers is revealed within the grain; (2) differential staining of the locus of the grain and the lamellae alternating with it in a small region around the longitudinal axis of the grain; (3) the simultaneous staining and separation of the grain into a cone-shaped peripheral portion and a spherical body containing the locus of the grain which emerges from it; and (4) differential staining of a ring or layer of substance around a spherical refractive body within the grain.

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