Abstract
The interrelationship between the pale‐yellow character and certain agronomic and chemical traits was studied in isogenic lines derived from five cultivars of flue‐cured tobacco (Nicotiana tabacum L.). Isogenic lines with the pale‐yellow character were compared with their green recurring parent and a green sister selection in each family. The pale‐yellow gene had little or no effect on days to flower or total alkaloid content. Slightly reduced leaf number and yield were associated with pale‐yellow lines in some families. A decrease in starch and sugar and an increase in alpha amino nitrogen were associated with the pale‐yellow character. The grade index of pale‐yellow lines was equal to or better than that of the recurrent parent or green sister selections.

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