Indirect Alkylation of CBA Mouse Liver DNA and RNA by Hydrazine In Vivo. A Possible Mechanism of Action as a Carcinogen23

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Abstract
Treatment of inbred CBA mice with hydrazine sulfate. a liver carcinogen. plus [14C]formate or l-[methyl-14C]methionine as a source of “C1” units, produced [14C]7-methylguanine (7-MG) in liver DNA and RNA. This methylated guanine was identified by its chromatographic characteristics in three different systems: on Sephadex G10, on Dowex 50, and by paper co-chromatography with authentic 7-MG. Mice that received [14C]formate alone showed no 7-MG in their DNA or RNA. In addition. mice treated with l-[methyl-14C]methionine alone showed no 7-MG in their DNA. These results suggest that “indirect” alkylation of nucleic acids is a mechanism of cancer initiation by hydrazine.