Liver and brain mitochondria

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Abstract
A medium based on the composition of the intracellular fluid of liver and brain was devised for use in the study of mitochondria. The rate of oxidation of various substrates is low, but may be increased 3- to 4-fold for liver and 2- to 3-fold for brain mitochondria by the addition of hexokinase and glucose, potato apyrase or 2:4-dinitrophenol. In the study of oxidative phosphorylation with hexokinase and glucose as trapping agents, the esterification of inorganic phosphate is linear at 37 degrees until more than 90% is removed. Liver mitochondria are stable during oxidation of pyruvate for at least 3 hours. The rate of oxidation of pyruvate by brain mitochondria decreases after 50-60 minutes. The addition of a variety of coenzymes does not influence this loss of activity.

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