GENETICS OF GLOSSINA MORSITANS MORSITANS (DIPTERA: GLOSSINIDAE). VI. MULTILOCUS COMPARISON OF THREE LABORATORY POPULATIONS

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Abstract
Three strains (Kariba, Handeni and ocra) of G. m. morsitans Westwood were examined by polyacrylamide gel electrophoresis to determine the extent of genetic similarity among the strains. Males were examined for 11 thoracic enzymes, 1 testicular enzyme and 1 midgut enzyme. Tetrazolium oxidase, Mn stimulated malate dehydrogenase, an .alpha.-glycerophosphate dehydrogenase, and testicular esterase were monomorphic. Variation was found in xanthine oxidase, aldehyde oxidase, octanol dehydrogenase, an .alpha.-glycerophosphate dehydrogenase, malate dehydrogenase, midgut alkaline phosphatase, 2 esterases, arginine phosphokinase and glucose-6-phosphate dehydrogenase. Loci for the latter 2 enzymes are on the X-chromosome; all others are on autosomes. Allele frequencies in the 3 strains indicated that the Kariba and ocra strains are more closely related to each other than either is to the Handeni strain. These genetic similarities are consistent with the geographic origins of the strains. The mean heterozygosity per locus was highest (16.7 and 16.0%) in the 2 strains (Kariba and ocra) which have the highest reproductive capacity under laboratory conditions, and lowest (7.3%) in the strain (Handeni) which has the lowest reproductive capacity.