Adsorption studies of human serum albumin, human γ-globulins, and human fibrinogen on the surface of P(S/PGL) microspheres

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Abstract
Adsorption of human serum albumin (HSA), human γ-globulins (γ G), and human fibrinogen (Fb) onto the surface of poly(styrene/α-t-butoxy-ω-vinylbenzyl-polyglycidol) microspheres (P(S/PGL)) with controlled fraction of polyglycidol in the interfacial layer was investigated. The microspheres were synthesized by the emulsifier-free radical copolymerization of styrene and α-tbutoxy-ω-vinylbenzyl-polyglycidol macromonomer (PGL). Macromonomers with number average molecular weights M n = 950 and 2700 were used in the syntheses. Fraction of polyglycidol in the microsphere surface layer was varied from 0.22 to 0.44, depending on the composition of the monomer feed. It was found that the maximal surface concentration of adsorbed proteins and the equilibrium constant of protein adsorption decreased with increased fraction of polyglycidol in the microsphere surface layer. For microspheres with the highest fraction of polyglycidol at the surface the maximal surface protein concentration was c. ten times lower and the ad...

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