Function of the Fibronectin-Binding Serum Opacity Factor ofStreptococcus pyogenesin Adherence to Epithelial Cells
Open Access
- 1 July 2004
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 72 (7) , 4302-4308
- https://doi.org/10.1128/iai.72.7.4302-4308.2004
Abstract
The serum opacity factor (SOF) ofStreptococcus pyogenesis a serotyping tool and pathogenesis factor. Using SOF-coated latex beads in cell adherence assays and antiserum directed against SOF inS. pyogenes-HEp-2 cell adherence inhibition experiments, we demonstrate SOF involvement in the fibronectin-mediated adherence ofS. pyogenesto epithelial cells. SOF exclusively targets the 30-kDa N-terminal region of fibronectin. The interaction revealed association and dissociation constants 1 order of magnitude lower than those of otherS. pyogenesfibronectin-binding proteins.Keywords
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