Description of a Nonlethal Herpes Simplex Virus Type 1 Glycoprotein D Deletion Mutant Affecting a Site Frequently Used for PCR
- 1 March 2000
- journal article
- research article
- Published by American Society for Microbiology in Clinical and Diagnostic Laboratory Immunology
- Vol. 7 (2) , 322-324
- https://doi.org/10.1128/cdli.7.2.322-324.2000
Abstract
We report a herpes simplex virus type 1 mutant which failed to amplify with a commonly used glycoprotein D primer set. The virus contained a nine-base deletion in the gene's 5′ nontranslated region. The altered amplicon was clearly distinguishable on a 4% high-resolution agarose gel.Keywords
This publication has 18 references indexed in Scilit:
- A comparison of virus isolation, indirect immunofluorescence and nested multiplex polymerase chain reaction for the diagnosis of primary and recurrent herpes simplex type 1 and type 2 infectionsJournal of Virological Methods, 1999
- The Etiology of Genital Ulcer Disease by Multiplex Polymerase Chain Reaction and Relationship to HIV Infection Among Patients Attending Sexually Transmitted Disease Clinics in Pune, IndiaSexually Transmitted Diseases, 1999
- Etiology of Genital Ulcers and Prevalence of Human Immunodeficiency Virus Coinfection in 10 US CitiesThe Journal of Infectious Diseases, 1998
- Molecular Methods for the Diagnosis of Genital Ulcer Disease in a Sexually Transmitted Disease Clinic Population in Northern Thailand: Predominance of Herpes Simplex Virus InfectionThe Journal of Infectious Diseases, 1998
- The role of herpes simplex virus glycoproteins in the virus replication cycle.1998
- Herpes simplex type 1 and genital herpes in Northern Ireland.1997
- Neuroinvasive properties of herpes simplex virus type 1 glycoprotein variants are controlled by the immune response.The Journal of Immunology, 1996
- Immunisation against HSV-1 keratitis with a synthetic gD peptideEye, 1995
- Encephalitis in immunocompetent patients due to herpes simplex virus type 1 or 2 as determined by type‐specific polymerase chain reaction and antibody assays of cerebrospinal fluidJournal of Medical Virology, 1993
- Herpes Simplex Virus Type-1 Glycoprotein D Gene: Nucleotide Sequence and Expression in Escherichia coliScience, 1982