Activity of FCE 22101 against methicillin-resistant Staphylococcus aureus and affinity for penicillin binding proteins
- 1 January 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Antimicrobial Chemotherapy
- Vol. 23 (suppl C) , 59-64
- https://doi.org/10.1093/jac/23.suppl_c.59
Abstract
The susceptibility of 47 clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) to FCE 22101, imipenem and methicillin was determined with Iso-Sensitest media, with or without NaCl and with incubation at 30° and 37°C and for 24 and 48 h. All strains had a MIC 8 mg/l of methicillin under at least one of the culture conditions. The MIC90 of FCE 22101 was 1 mg/l, and that of imipenem 16 mg/l. The affinity of FCE 22101 for the penicillin-binding proteins (PBPs) of five clinical isolates of MRSA and S. aureus 13136 p − m + was examined in envelope preparations and whole cell assay under the growth conditions listed above. PBP 2′ was detected in all MRSA, and the clinical isolates had an I 50 of 4 mg/l FCE 22101. These in-vitro data suggest that FCE 22101 may be active against MRSA in clinical use.Keywords
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