A COMPARISON OF THE LEUKOCYTE AGGLUTINATION AND MIXED AGGLUTINATION TECHNIQUES FOR DETECTING HUMAN TISSUE ISOANTIGENS

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Abstract
SUMMARY The tissue isoantigenic mosaics of five human donors were determined by testing a common panel of human isoantisera, with the use of two techniques: agglutination of peripheral blood leukocytes, and mixed agglutination with tissue culture cells derived from kidney or skin. The antisera used were from multiparous women or immunized volunteers and were known to have leukocyte isoantibodies. Chi-square values were calculated on the reactions of the panel with cells from each of the donors by the two methods. All but one of the values showed a significant association at the 1% level, the other value at the 5% level.