The effect of a ribonuclease inhibitor from human placenta on the in vitro synthesis of human placental proteins
- 7 February 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 152 (1) , 35-38
- https://doi.org/10.1016/0014-5793(83)80476-1
Abstract
Addition of a ribonuclease inhibitor (10 μg/ml) from human placenta caused 2–3 fold increase of [3H]leucine incorporation in the wheat germ extract as directed by human placental poly (A)‐mRNA. Analysis of the translated products by sodiumdodecylsulfate/polyacrylamide gel electrophoresis/fluorography revealed that the inhibitor preferentially increased the yields of the larger proteins, particularly those of larger than M r 40 000. In the presence of the inhibitor, yields of two placental proteins (human placental lactogen and human chorionic gonadotropin) were increased about 70–80% as detected by immunoprecipitation with specific homologous antisera. The method provided an improvement of translation system for studying biosynthesis of other human placental proteins.Keywords
This publication has 15 references indexed in Scilit:
- Effect of human placental ribonuclease inhibitor in cell-free ribosomal RNA synthesisBiochemical and Biophysical Research Communications, 1981
- Synthesis and core glycosylation of the α subunit of human chorionic gonadotropin in Xenopus oocytesFEBS Letters, 1980
- The new placental proteinsPlacenta, 1980
- Role of mammalian RNase inhibitor in cell-free protein synthesisProceedings of the National Academy of Sciences, 1979
- Isolation, cloning and sequence analysis of the cDNA for the α-subunit of human chorionic gonadotropinNature, 1979
- Efficient Translation of Tobacco Mosaic Virus RNA and Rabbit Globin 9S RNA in a Cell-Free System from Commercial Wheat GermProceedings of the National Academy of Sciences, 1973
- Quantitative Studies of Human Placenta II.Neonatology, 1973
- Purification of Biologically Active Globin Messenger RNA by Chromatography on Oligothymidylic acid-CelluloseProceedings of the National Academy of Sciences, 1972
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- Measurement of the incorporation of radioactive amino acids into protein by a filter-paper disk methodArchives of Biochemistry and Biophysics, 1961