OXYTOCIN RELEASE FROM NEUROHYPOPHYSIS EVOKED BY PREGANGLIONIC STIMULATION OF THE RAT SUPERIOR CERVICAL-GANGLION

  • 1 January 1982
    • journal article
    • research article
    • Vol. 79  (3) , 385-392
Abstract
In male rats under urethane/chloralose anesthesia the pituitary gland was exposed by transpharyngeal approach. The following methods were applied: posterior pituitary lobe incubation in situ with the anterior lobe removed and the posterior lobe elevated, so that only the vessels between the lobe and the meninges are broken, and continuous exchange of the incubation fluid; removal of the entire pituitary and continuous exchange of the fluid washing of the cut proximal end of the pituiary stalk. Twenty to 30 min samples of incubation or washing fluid were collected for 3-5 h and oxytocin was assayed according to Van Dongen and Hays. Preganglionic stimulation of the superior cervical ganglion caused significant increase (.apprx. 25 times) of otytocin release from the posterior pituitary lobe. Preganglionic stimulation of the superior cervical ganglion did not alter oxytocin content in the fluid washing of the cut proximal end of the pituitary stalk. The sympathetic neurons of the superior cervical ganglia could evoke oxytocin release from the neurohypophysis but they do not change its outflow from the hypothalamus to the neurohypophysis.