Structural basis of ubiquitin recognition by mammalian Eap45 GLUE domain
- 22 October 2006
- journal article
- research article
- Published by Springer Nature in Nature Structural & Molecular Biology
- Vol. 13 (11) , 1031-1032
- https://doi.org/10.1038/nsmb1163
Abstract
ESCRT-II, a complex that sorts ubiquitinated membrane proteins to lysosomes, localizes to endosomes through interaction between the Vps36 subunit's GLUE domain and phosphatidylinositides (PIs). In yeast, a ubiquitin (Ub)-interacting NZF domain is inserted in Vps36 GLUE, whereas its mammalian counterpart, Eap45 GLUE, lacks the NZF domain. In the Eap45 GLUE–Ub complex structure, Ub binds far from the proposed PI-binding site of Eap45 GLUE, suggesting their independent binding.Keywords
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