17-Oxidoreduction of 17β-Estradiol, Estrone and Their 3-Sulfates by Kidney Slices from Guinea Pig and Human

1 December 1975

journal article
Published by Canadian Science Publishing in Canadian Journal of Biochemistry

Vol. 53 (12) , 1333-1336
https://doi.org/10.1139/o75-181

Abstract

Slices of whole kidney and kidney cortex from the female guinea pig catalyzed a marked reduction of estrone 3-sulfate (E₁3S) and estrone (E₁) to 17β-estradiol 3-sulfate (E₂3S) and 17β-estradiol (E₂), respectively, as well as the reverse (dehydrogenation) reactions. Slices of medulla did not appear active in E₂3S–E₁3S interconversion but did possess the ability to interconvert E₂ and E₁, besides possessing considerable sulfatase activity. The use of [³H-³⁵S]E₁3S and [³H-³⁵S]E₂3S as substrates, together with a demonstrated lack of estrogen sulfate synthesis by the tissue slices, provided ample evidence that the intact sulfates were involved in direct oxidoreduction. Slices of human kidney cortex catalyzed the reduction of E₁3S to a very limited extent. Slices of whole kidney and of cortex from guinea pig formed small amounts of estrogen glucuronide(s).

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