Zymosan-Stimulated Production of Phosphatidic Acid by Macrophages: Relationship to Release of Superoxide Anion and Inhibition by Agents That Increase Intracellular Cyclic AMP
- 1 May 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Leukocyte Biology
- Vol. 41 (5) , 450-453
- https://doi.org/10.1002/jlb.41.5.450
Abstract
Murine peritoneal macrophages (mΦ) respond to unopsonized zymosan with the production of superoxide anion (O2 -). We investigated the involvement of phospholipid turnover in the transduction mechanism for this phenomenon. Zymosan-stimulated mΦ produced increased amounts of phosphatidic acid (PA); the increase was first detected at 1.5 min and continued for 10 min of incubation. Production of O2 - was not detected until between 2 to 4 min after stimulation, and continued to increase through 60 min. Inhibition experiments suggested that these two processes were linked. Theophylline (theo)/ dibutyrylcyclic AMP (dbcAMP) and theo/prostaglandin E2 (PGE2) inhibited O2 - production at every time point (79% and 80% inhibition, respectively, at 4 min). Corresponding inhibition of PA production was also achieved at every time point (85% by theo/dbcAMP; 67% by theo/PGE2 at 4 min). These results are compatible with a role for phospholipid remodeling in the transduction process associated with the respiratory burst. Results suggest that the phospholipid species could be phosphatidylcholine (PC) as well as phosphatidylinositol (PI).Keywords
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