Estimating Somatic Cells in Milk Samples by the Membrane-Filter-DNA Procedure
- 1 January 1977
- journal article
- research article
- Published by Elsevier in Journal of Food Protection
- Vol. 40 (1) , 32-38
- https://doi.org/10.4315/0362-028x-40.1.32
Abstract
Further developments in the membrane-filter DNA procedure for determining the somatic cell content of milk samples are described. A statistical experiment has been done comparing the membrane-filter test to the direct microscopic somatic cell count. Under conditions of the assay a relationship of 0.093 absorbance unit per million cells was obtained at a wavelength of 490 nm. The membrane filter technique is more precise than the direct microscopic count and is not subject to techician bias. A method is described for standardizing the procedure between and within laboratories based on use of lyophilized bovine somatic cells trapped on membrane filters as a calibration device.This publication has 3 references indexed in Scilit:
- DEVELOPMENT AND EVALUATION OF THE DIRECT MICROSCOPIC SOMATIC CELL COUNT (DMSCC) IN MILK1Journal of Milk and Food Technology, 1969
- An ultramicro technique for the determination of deoxypentose nucleic acidArchives of Biochemistry and Biophysics, 1956
- A MICROCHEMICAL DETERMINATION OF DESOXYRIBONUCLEIC ACIDJournal of Biological Chemistry, 1952