INVESTIGATIONS ON ISOLATED ISLETS OF LANGERHANS INVITRO .13. EXPERIMENTS CONCERNING PREPARATION CONDITIONS WITH COLLAGENASE

  • 1 January 1976
    • journal article
    • research article
    • Vol. 67  (1) , 67-78
Abstract
During the digestion of pancreatic pieces with collagenase for preparation of isolated [rat] islets the enzymes in incubation medium (collagenolytic and/or proteolytic) can alter the secretion behavior of .alpha.- and .beta.-cells. Insulin release after such an enzymatic attack is characterized by an enhanced basal secretion and a diminished and delayed glucose response. Overdigestion results in a decreased glucagon secretion in response to arginine, a diminished insulin content and a decreased thiol-protein-disulfide-oxidoreductase activity of the islets. Increased [bovine] albumin concentrations did not prevent the collagenase effect.

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