Role of glycolysis in the tension development under anoxia in guinea pig taenia coli.

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Abstract
The role of glycolysis in the tension development under anoxic conditions in a high-K medium was studied in the intestinal smooth muscle of guinea pig tenia coli. After exposure to the high-K medium (isotonic, 60 mM) under normal O2 for 30 min, the muscles were exposed to a high-K medium bubbled with N2 gas. The tonic contraction decreased gradually to .apprx. 10% of the original level. Glucose was then cumulatively added to the high-K medium under anoxia. The maximum tension was observed following the addition of the higher concentrations of glucose. The muscle tension which developed in the high-K medium with a high concentration of glucose under anoxia was dependent on the external Ca2+ and was inhibited by IAA. The addition of glucose to a high-K medium under anoxia also increased lactate release from the muscle. Pretreatment with 1 mM IAA decreased the lactate release from the muscle. In a Ca2+-free medium under anoxia, the addition of glucose did not increase the muscle tension, although there was a significant increase in the lactate release. The smooth muscle of tenia coli develops tension utilizing energy produced by the glycolytic pathway under anoxia in a high-K medium.