Detection of Anti-Phage Antibody by Passive Hemagglutination

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Abstract
A passive hemagglutination test for detecting and measuring antibody activity against phage T2 was developed. Phage treated with the detergent sodium dodecyl sulfate under specified conditions of concentration of reagents and pH effectively sensitized tanned sheep red cells to agglutination by antibodies specific for T2 phage. It was shown that several phage antigens, including those of the tail fibers that are implicated in neutralization tests, are absorbed to the tanned cells. The sensitivity of this test for detecting antibodies is shown to match or exceed that of the neutralization test, presumably because it is more comprehensive in its detection of the reactions between the several phage antigens and their respective antibodies.

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