1H and 13C NMR analysis of brassinosteroids

Abstract
The 1H and 13C NMR spectra of the two phytohormones, brassinolide and 24‐epi‐brassinolide, and the two synthetic analogues 22,23,24‐trisepi‐brassinolide and (22S,23S)‐28‐homo‐brassinolide, were assigned using 1D and 2D NMR techniques. Independent assignment strategies are presented for the 1H and 13C chemical shifts of the steroidal side‐chain.