Effect of chlorhexidine upon 32P release and cell viability in Escherichia coli

Abstract
The amount of the cold trichloroacetic acid soluble fraction (metabolic pool), expressed as a percentage of its 32P content, released from labelled cells of Escherichia coli treated with chlorhexidine has been compared with the percentage of cells killed by the chlorhexidine. Treatment was with amounts varying from 0–64 μg/ml for 5 min at 20°. Approximately 100% of the cells were killed and 100% of the metabolic pool released by treatment with 64μg/ml of chlorhexidine. At 4, 8 and 16 μg/ml the percentage of cells killed by chlorhexidine is significantly lower than the percentage of the metabolic pool released.

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