Hepatocyte Chromosomal Non‐histone Proteins in Developing Rats

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Abstract
Rat hepatocytes taken at different stages of the perinatal period were partially purified. On sodium dodecylsulfate polyacrylamide gel electrophoresis structures of chromosomal non-histone proteins varied during development. Chromosomal phosvitin kinase activity strongly increased during the last days of fetal life; it strongly decreased just after birth and increased again for a short time, while the cytosol phosvitin kinase increased more significantly after birth. Chromosomal non-histone proteins prepared at various stages were incubated with [.gamma.-32P]ATP and resolved on polyacrylamide gel. Incorporation was very low in samples taken at the 15th day of fetal life. A dramatic increase was observed at the 17th day. Incorporation strongly decreased in samples taken thereafter and was negligible in proteins from adult rats. The variations in protein kinase and in 32P incorporation into non-histone proteins were correlated with the pattern of appearance of enzymes in the perinatal period, with cell growth and with hormone-induced maturation.