Demonstration of Ferritin‐Labelled Antibodies Bound to Human Erythrocytes Fixed with Glutaraldehyde1

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Abstract
Summary: In this immuno‐electron microscopic study, the capability of the combining antibody and the agglutinability was examined in glutaraldehyde‐fixed erythrocytes.Glutaraldehyde‐fixed human red cells preserved a strong ability to bind specific antibodies as unfixed cells, even though they decrease in agglutinability.As the fixed cells were separated from one another, even when were agglutinating, the localization of antibodies on a cell in the fixed cells was very clear. The number of A antigen sites per red ceil of different phenotypes was estimated individually as each values of single cell but not as mean value of many cells.It was confirmed that the ability of the combining antibody varied greatly from cell to cell in the same blood of umbilical cord.