Isolation of Histone-Like Proteins from Mitochondria of Bovine Heart

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Abstract
Two methods for isolating and purifying histone-like proteins from mitochondria of bovine heart are described. In the first, a sonicated extract of the mitochondria was fractionated in three chromatography steps, including affinity chromatography on DNA-cellulose, to purify a protein that resembles very closely the histone-like protein (HM) of yeast mitochondria. In the second method, an acid extract of the heart mitochondria was the starting material; two other histone-like proteins were separated. Thus, as in mitochondria of Xenopus laevis. several histone-like proteins are present in mitochondria of bovine heart.