Characterization of class II histocompatibility antigens in pigs

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Abstract
The D region of the swine lymphocyte antigen complex in the pig was studied by immunochemical and sequential immunoprecipitation techniques and the redistribution of membranous antigens (capping). The molecules identified by the anti-Ia sera were solubilized by NP 40, purified on lectin and precipitated. Polyacrylamide gel electrophoresis under dissociating conditions shows that these molecules are made up of 2 chains whose MW are 32,000 and 26,000 daltons, respectively. Sequential immunoprecipitation and capping experiments indicate that 2 distinct types of Ia molecules exist. At least a part of the nylon-wood-adherent [peripheral blood] lymphocyte population expresses both molecule types.